Mouse FST / Follistatin ( FS288 ) Protein

20.03.2013

Sino Biological Inc. liefert Mouse FST / Follistatin ( FS288 ) Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse FST / Follistatin ( FS288 ) Protein lesen Sie bitte: http://www.sinobiological.com/Follistatin-Protein-a-2426.html

Produkt-Information von FST / Follistatin ( FS288 ) Protein

Synonym	AL033346
Protein Construction	A DNA sequence encoding the mouse FST (P47931) (Met 1-Asn 317) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Mouse
Expression Host	CHO Stable Cell

QC Testing von FST / Follistatin ( FS288 ) Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: FST protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly 30
Molecular Mass	The recombinant mouse FST comprises 528 amino acids and has a predicted molecular mass of 58.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 66 kDa due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von FST / Follistatin ( FS288 ) Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von FST / Follistatin ( FS288 ) Protein

Follistatin, also known as Activin-binding protein and FST, is a secreted protein. Follistatin / FST is an autocrine glycoprotein that is expressed in nearly all tissues of higher animals. Follistatin / FST contains 3 follistatin-like domains, 3 Kazal-like domains and 1 TB (TGF-beta binding) domain. Follistatin / FST is produced by folliculostellate (FS) cells of the anterior pituitary. FS cells make numerous contacts with the classical endocrine cells of the anterior pituitary including gonadotrophs. Isoform 1 of Follistatin / FST is the predominant isoform in serum but is undetectable in follicular fluid. Follistatin / FST is a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). Follistatin / FST binds directly to activin and functions as an activin antagonist. Follistatin / FST was initially isolated from follicular fluid and was identified as a protein fraction that inhibited follicle-stimulating hormone (FSH) secretion from the anterior pituitary, and so was known as FSH-suppressing protein (FSP). Follistatin / FST and BMPs are known to play a role in folliculogenesis within the ovary. The main role of Follistatin / FST in the oestrus/menstrus ovary appears to be progression of the follicle from early antral to antral/dominant, and importantly the promotion of cellular differentiation of the estrogen producing granulosa cells (GC) of the dominant follicle into the progesterone producing large lutein cells (LLC) of the corpus luteum.

References

Tortoriello DV. et al., 2001,Endocrinology. 142 (8): 3426-34.
Thompson TB. et al., 2005, Dev Cell. 9 (4): 535-43.
Lambert-Messerlian G. et al.,2007,Pediatr Dev Pathol. 10 (6): 436-45.
Walsh S. et al.,2007, J Appl Physiol. 102(6): 2142-8.
Nakatani M. et al.,2008, FASEB J. 22 (2): 477-87.

Cytokine

TGF-beta