Mouse E-selectin / ELAM-1 / CD62E Protein (His Tag)

20.03.2013

Sino Biological Inc. liefert Mouse E-selectin / ELAM-1 / CD62E Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse E-selectin / ELAM-1 / CD62E Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/E-Selectin-CD62E-Protein-Antibody-a-319.html

Produkt-Information von E-selectin / ELAM-1 / CD62E Protein

Synonym	SELE
Protein Construction	A DNA sequence encoding the mouse SELE (Met1-Pro564) was expressed with a C-terminal polyhistidine tag.
Source	Mouse
Expression Host	Human Cells

QC Testing von E-selectin / ELAM-1 / CD62E Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: E-selectin protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Trp 29
Molecular Mass	The recombinant mouse SELE comprises 547 amino acids and has a predicted molecular mass of 60 kDa. The apparent molecular mass of the protein is approximately 70-80 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von E-selectin / ELAM-1 / CD62E Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von E-selectin / ELAM-1 / CD62E Protein

E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed at relatively high density on the surface of cultured endothelial cells that have been activated by proinflammatory cytokines, such as IL-1β, TNF-α, or bacterial endotoxin. E-Selectin/CD62e exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-Selectin/CD62e recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-Selectin/CD62e supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. In addition, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.

References

Hession C. et al., 1990, Proc. Natl Acad Sci. 87: 1673-7.
Philips ML. et al., 1990, Science. 250: 1130-2.
Luo J. et al., 1999, Blood. 93: 3785-91.
Hu Y. et al., 2001, J Biol Chem. 276: 48549-53.
Bevilacqua MP. et al., 1993, J Clin Invest. 91: 379-84.