Mouse CNTN5 / Contactin-5 Protein (His Tag)

24.05.2013

Sino Biological Inc. liefert Mouse CNTN5 / Contactin-5 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse CNTN5 / Contactin-5 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/Contactin-5-CNTN5-Protein-Antibody-a-124.html

Produkt-Information von CNTN5 / Contactin-5 Protein

Synonym	CNTN5
Protein Construction	A DNA sequence encoding the mouse CNTN5 (P68500) (Met1-Gln1058) was expressed with a C-terminal polyhistidine tag.
Source	Mouse
Expression Host	Human Cells

QC Testing von CNTN5 / Contactin-5 Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: CNTN5 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Leu 24
Molecular Mass	The recombinant mouse CNTN5 comprises 1046 amino acids and has a predicted molecular mass of 115.2 kDa. The apparent molecular mass of the protein is approximately 118 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CNTN5 / Contactin-5 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibun von CNTN5 / Contactin-5 Protein

Contactin-5 (CNTN5), also known as NB-2, is an membrane-anchored neuronal protein and also an adhesion molecule. CNTN5 is one of the neural recognition molecules in the contactin subgroup, which belongs to the immunoglobulin superfamily. CNTN5 shared 46, 43, 55 and 55% identities with CNTN1,CNTN2,CNTN3,CNTN4 respectively at the amino acid sequence level. Expression of NB-2 mRNA was restricted to cerebrum, cerebellum and was hardlty detectable, if any, in spinal cord. In humans, two isoforms have been identified. The long isoform of human NB-2 consists of 1100 amino acids residues and the short isoform lacks 74 amino acid residues between residues 19 and 93 of the long isoform. CNTN5 may play a role in the developing nervous system. NB-2 promoted neurite outgrowth of the cerebral cortical neurons but not the hippocampal neurons. CNTN5-deficient mice demonstrate impaired neuronal activity in the auditory system, suggesting that it plays specific roles in the development and/or maturation of the regions.

References

Soumya Raychaudhuri. et al., 2009, PLoS Genetics. 5 (6): e1000534.
Yoshimasa Kamei. et al., 2000, Genomics. 69: 113-9.
Junko Ogawa. et al., 1996, Neuroscience Letters. 218: 173-6.
P S Walmod. et al., 2007, Handbook of Neurochemistry and Molecular Neurobiology. 105.
Junko Ogawa. et al., 2001, Journal of Neuroscience Research. 65:100-10.