Cynomolgus ACE2 / ACEH Protein

22.05.2013

Sino Biological Inc. liefert Cynomolgus ACE2 / ACEH Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus ACE2 / ACEH Protein lesen Sie bitte: http://www.sinobiological.com/ACE2-Protein-a-709.html

Produkt-Information von ACE2 / ACEH Protein

Synonym	ACE2
Protein Construction	A DNA sequence encoding the cynomolgus ACE2 (B6DUF3) (Met1-Val739) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von ACE2 / ACEH Protein

Purity	(92.8+4.3)% as determined by SDS-PAGE	SDS-PAGE: ACE2 protein
Endotoxin:	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gln 18
Molecular Mass	The recombinant cynomolgus ACE2 is a disulfide-linked homodimer. The reduced monomer comprises 963 amino acids and has a calculated molecular mass of 110.7 KDa.The apparent molecular mass of the protein is approximately 112 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ACE2 / ACEH Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ACE2 / ACEH Protein

Angiotensin-converting enzyme 2, also known as ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15, ACEH and ACE2, is a single-pass type I membrane protein which belongs to the peptidase M2 family. ACEH / ACE2 is identified as a zinc metalloprotease structurally consisting of a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACEH / ACE2 is expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. It is expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). It is expressed in heart, kidney, testis, and gastrointestinal system. ACEH / ACE2 is a carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. It is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACEH / ACE2 may be an important regulator of heart function. It may also serve as functional receptor for the spike glycoprotein of both coronaviruses. Coronaviruses primarily cause respiratory and enteric diseases in mammals and birds. Coronavirus symptoms include rhinorrhea, sneezing, cough, nasal obstruction, bronchitis and so on. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Novel coronavirus 2012 (hCoV-EMC) does not use the human receptor protein ACE2. The receptor used by HCov-EMC has not been identified. Increased expression of ACE2 increases infection rate.

References

Donoghue M. et al., 2000, Circ Res. 87: E1-E9.
Carey RM. et al., 2004, Hypertension. 43: 943-4.
Crackower MA. et al., 2002, Nature. 417: 822-8.
Tipnis SR. et al., 2000, J Biol Chem. 275: 33238-43.
Oudit GY. et al., 2003, Trends Cardiovasc Med. 13: 93-101.
Wang S. et al., 2008, Virus Res. 136: 8-15.