Mouse CD3D & CD3E Heterodimer Protein (Flag & His Tag)

21.05.2013

Sino Biological Inc. liefert Mouse CD3D & CD3E Heterodimer Protein (Flag & His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse CMouse CD3D & CD3E Heterodimer Protein (Flag & His Tag) lesen Sie bitte: http://www.sinobiological.com/CD3D-CD3E-Heterodimer-Protein-g-11735.html

Produkt-Information von CD3D & CD3E / CD3 delta & CD3 epsilon Heterodimer Protein

Synonym	CD3D&CD3E
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1- Ala 105) of mouse CD3D (P04235) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1- Asp 108) of mouse CD3E (P22646) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
Source	Mouse
Expression Host	Human Cells

QC Testing von CD3D & CD3E / CD3 delta & CD3 epsilon Heterodimer Protein

Purity	(42.3 + 45.3) % as determined by SDS-PAGE	SDS-PAGE: CD3D & CD3E protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Phe 22& Asp 22
Molecular Mass	The recombinant heterodimer of mouse CD3D/CD3E comprises 254 (126+128) amino acids and has a calculated molecular mass of 29.2 (14.3+14.9) KDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 20 and 22 KDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CD3D & CD3E / CD3 delta & CD3 epsilon Heterodimer Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CD3D & CD3E / CD3 delta & CD3 epsilon Heterodimer Protein

T-cell surface glycoprotein CD3 delta/epsilon chain, also known as CD3D and CD3E respectively, are single-pass type I membrane proteins. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). The immunofluorescence defined that CD3 epsilon(CD3E0) was strongly stained bound to either CD 3 gamma or CD 3 delta. The recognition by this panel of anti-CD3 antibodies of CD3-gamma/ epsilon and CD3-delta/ epsilon complexes and not of CD3-epsilon alone was assessed by immunoprecipitation.

References

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Arnett KL. et al., 2004, Proc Natl Acad Sci. 101: 16268-73.
Kjer-Nielsen L. et al., 2004, Proc Natl Acad Sci. 101: 7675-80.
Fischer A. et al., 2005, Curr Opin Allergy Clin Immunol. 5 (6): 491-5.
Holst J. et al., 2008, Nature immunology. 9 (6): 658-66.
Salmerón A. et al., 1991, J Immunol. 147 (9): 3047-52.5.