IL10 / Interleukin-10 Protein (Native)

21.05.2013

Sino Biological Inc. liefert IL10 / Interleukin-10 Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über IL10 / Interleukin-10 Protein (Native) lesen Sie bitte: http://www.sinobiological.com/IL-10-Protein-a-1446.html

Produkt-Information von IL10 / Interleukin-10 Protein

Synonym	IL10
Protein Construction	A DNA sequence encoding human IL10 (NP_000563.1) (Ser 19-Asn 178) was expressed.
Source	Human
Expression Host	E.coli

QC Testing von IL10 / Interleukin-10 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: IL10 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ser 19
Molecular Mass	The recombinant human GADD45G consists of 160 amino acids and predicts a molecular mass of 18.6 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 20mM NaCl, pH 8.7. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von IL10 / Interleukin-10 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von IL10 / Interleukin-10 Protein

Interleukin-10, also known as cytokine synthesis inhibitory factor, CSIF and IL-10, is a secreted protein which belongs to the IL-10 family. Interleukin-10 / IL-10 is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. It can also be produced by monocytes upon PD-1 triggering in this cells. Interleukin-10 / IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Interleukin-10 / IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies suggested the function of Interleukin-10 / IL-10 as an essential immunoregulator in the intestinal tract. Patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin-10, showing the importance of interleukin-10 for counteracting excessive immunity in the human body.

References

Gesser B. et al., 1997, Proc Natl Acad Sci. 94: 14620-5.
Eskdale J. et al.,1997, Immunogenetics. 46 (2): 120-8.
Yoon SI. et al., 2005, Structure. 13: 551-64.
Braat H. et al.,2006, Clin Gastroenterol Hepatol. 4 (6): 754-9.
Grimbaldeston MA. et al., 2007, Nat. Immunol. 8 (10): 1095-104.
Elias A. et al., 2009, Nature Medicine. 2010: 452-9.