Mouse BACE2 / Beta secretase 2 Protein (His Tag)

28.11.2012

Sino Biological Inc. liefert Mouse BACE2 / Beta secretase 2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse BACE2 / Beta secretase 2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/BACE2-Beta secretase 2-Protein-g-9921.html

Produkt-Information von BACE2 / Beta secretase 2 Protein

Synonym	BACE2
Protein Construction	A DNA sequence encoding the mouse BACE2 (Q9JL18) (Met1- Pro462) was expressed with a C-terminal polyhistidine tag.
Source	Mouse
Expression Host	Human Cells

QC Testing von BACE2 / Beta secretase 2 Protein

Purity	>95% as determined by SDS-PAGE	SDS-PAGE: BACE2 protein
Bio-activity	Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010). The specific activity is >50 pmoles/min/μg.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 20
Molecular Mass	The recombinant mouse BACE2 comprises 454 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von BACE2 / Beta secretase 2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von BACE2 / Beta secretase 2 Protein

BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It can be detected in in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). BACE2 is a close homolog of BACE1, a protease known to be an important enzyme involved in the cellular pathways that some believe lead to Alzheimer's disease. BACE2 is responsible for the proteolytic processing of the amyloid precursor protein (APP). BACE2 has been shown to interact with GGA1 and GGA2. Overexpression of BACE2 by lentivirus markedly reduced amyloid β protein production in primary neurons.

References

Hussain I. et al., 2001, J Biol Chem. 276 (26): 23322-8.
Solans A. et al., 2000, Cytogenet Cell Genet. 89 (3-4): 177-84.
Hussain I. et al., 2001, Mol Cell Neurosci. 16 (5): 609-19.