Mouse AGRP Protein

27.03.2013

Sino Biological Inc. liefert Mouse AGRP Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse AGRP Protein lesen Sie bitte: http://www.sinobiological.com/AGRP-Protein-a-723.html

Produkt-Information von AGRP Protein

Synonym	AGRP, Agrt, Art
Protein Construction	A DNA sequence encoding the mouse AGRP (Met1-Thr131) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Mouse
Expression Host	Human Cells

QC Testing von AGRP Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: AGRP protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Val 21
Molecular Mass	The recombinant mouse AGRP /Fc is a disulfide-linked homodimer. The reduced monomer comprises 352 amino acids and has a predicted molecular mass of 39.4 KDa. The apparent molecular mass of the protein is approximately 41 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von AGRP Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von AGRP Protein

Agouti-related protein or Agouti-related peptide (AgRP) is a neuropeptide predominantly produced in the brain (in the arcuate nucleus of the hypothalamus) by the AgRP / NPY neuron, and functions in the hypothalamic control of feeding behavior. As one of the most potent and long-lasting of appetite stimulators, AgRP increases appetite and decreases metabolism and energy expenditure, and thus regulates the development of obesity. AgRP has been identified as one of the two naturally occurring inverse agonists of melanocortin receptors, specifically MC3-R and MC4-R,which belongs to the G protein-coupled receptor (GPCR )family. This endogenous antagonist not only antagonizes the action of melanocortin agonists, such as α-melanocyte stimulating hormone (α-MSH), but also decreases the cAMP production by the affected cells and is involved in intracellular calcium regulation. Meanwhile, Agouti has been shown to regulate skin pigmentation by inhibiting MC1R signaling in melanocytes. In addition, the appetite stimulating effects of AgRP are inhibited by the hormone leptin secreted by adipocytes. Upregulated expression of AgRP is observed in obesity and diabetes, and genetically AgRP polymorphisms are associated with susceptibility to anorexia nervosa.

References

Ollmann MM. et al., 1997, Science. 278: 135-8.
Yang Y. et al., 2003, Mol Pharmacol. 64: 94-103.
Breit A. et al., 2006, J Biol Chem. 281: 37447-56.
Katsuki A. et al., 2001, J Clin Endocr Metab. 86: 1921-4.
Graham M. et al., 1997, Nat Genet. 17: 273-4.
Shinyama H. et al., 2003, Endocrinology. 144: 1301-14.