NAA10 / ARD1A Protein

27.03.2013

Sino Biological Inc. liefert NAA10 / ARD1A Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über NAA10 / ARD1A Protein lesen Sie bitte: http://www.sinobiological.com/ARD1-Protein-Antibody-a-5724.html

Produkt-Information von NAA10 / ARD1A Protein

Synonym	ARD1A,NAA10,ARD1,TE2
Protein Construction	A DNA sequence encoding the human ARD1A (P41227) (Met1-Ser 235) was expressed and purified.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von NAA10 / ARD1A Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: NAA10 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly
Molecular Mass	The recombainant human ARD1A consists of 237 amino acids and predicts a molecular mass of 26.6 KDa. It migrates as an approximately 31 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von NAA10 / ARD1A Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von NAA10 / ARD1A Protein

ARD1 is a member of the 20-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 403-574), and a 46-kDa N-terminal domain (amino acids 1-402). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating type locus HML, for sporulation, and for entry into G0. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-110) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.

References

Hartley JL. et al., 2001, Genome Res. 10 (11): 1788-95.
Simpson JC. et al., 2001, EMBO Rep. 1 (3): 287-92.
Bae MK. et al., 2003, Cell. 111 (5): 709-20.

ARD1 related areas, pathways, and other information