Mouse ACO2 Protein (GST Tag)

03.05.2012

Produkt-Information von ACO2 Protein

Synonym	Aco-2, Aco3, D10Wsu183e
Protein Construction	A DNA sequence encoding the mouse ACO2 (Q99KI0) (Gln 28-Gln 780) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Mouse
Expression Host	Baculovirus-Insect cells

QC Testing von ACO2 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: ACO2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant mouse ACO2/GST chimera consists of 990 amino acids and has a calculated molecular mass of 110 kDa. The recombinant protein migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, 10% gly, 0.5mM GSH, pH 8.0 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ACO2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ACO2 Protein

Aconitase employs a cluster to catalyze the stereospecific dehydration-rehydration of citrate to isocitrate via cis-aconitase in the second and third steps of the Krebs cycle. The protein contains four domains. Residues from all four domains contribute to the active site region, which is defined by the Fe[BOND]S cluster. Three of these side chains reside on a three turn 310 helix in the first domain. Diseases relevant to aconitase are aconitase deficiency, Friedreich's ataxia (FRDA), and diabetes. Aconitase deficiency is caused by mutation in the gene for iron-sulfur cluster scaffold protein, which helps to form the Fe-S cluster. FRDA is caused when the Fe-S proteins in aconitase and succinate dehydrogenase have decreased activity. ACO2 is the mitochondrial form of aconitase different from the soluble form ACO1.

References

Robbins AH. et al., 1989, Proteins. 5 (4): 289-312.
Lauble H. et al., 1992, Biochemistry. 31 (10): 2735-48.
Robbins AH. et al., 1989, Proc Natl Acad Sci. 86 (10): 3639-43.

Sino Biological Inc. liefert Mouse ACO2 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Mouse ACO2 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/ACO2-Protein-g-8836.html

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
ACO2	Human	ACO2 Protein, Recombinant, with GST Tag	10958-H20B
ACO2	Mouse	ACO2 Protein, Recombinant, with GST Tag	51015-M20B