MOBKL1A / MOB1B Protein (GST Tag)

22.04.2013

Sino Biological Inc. liefert MOBKL1A / MOB1B Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über MOBKL1A / MOB1B Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/MOB1B-MOBKL1A-Protein-Antibody-a-5852.html

Produkt-Information von MOBKL1A / MOB1B Protein

Synonym	MOBKL1A, MOB4A, MOB1B
Protein Construction	A DNA sequence encoding the human MOBKL1A (Q7L9L4) (Met1-Arg216) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von MOBKL1A / MOB1B Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: MOBKL1A protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human MOBKL1A /GST chimera consists of 450 amino acids and has a predicted molecular mass of 52.3 kDa. It migrates as an approximately 43-48 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von MOBKL1A / MOB1B Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MOBKL1A / MOB1B Protein

MST1 and MST2 are the mammalian Ste20-related protein kinases most closely related to Drosophila Hippo, a major regulator of cell proliferation and survival during development. Overexpression of MST1 or MST2 in mammalian cells is proapototic. MST1 and MST2 activity increases during mitosis, especially in nocodazole-arrested mitotic cells, where these kinases exhibit both an increase in both abundance and activation. MST1 and MST2 also can be activated nonphysiologically by okadaic acid or H2O2. The MOB1B and MOBKL1B polypeptides, homologs of the Drosophila MATS polypeptide, are identified as preferred MST1/MST2 substrates in vitro and are phosphorylated in cells in an MST1/MST2-dependent manner in mitosis and in response to okadaic acid or H2O2. MST1/MST2-catalyzed MOB1B/MOBKL1B phosphorylation alters the ability of MOB1B/MOBKL1B to bind and regulate downstream targets such as the NDR-family protein kinases. Thus, MOB1B/MOBKL1B phosphorylation in cells promotes MOB1B/MOBKL1B binding to the LATS1 kinase and enables H2O2-stimulated LATS1 activation loop phosphorylation. Most importantly, replacement of endogenous MOB1B/MOBKL1B by a nonphosphorylatable mutant is sufficient to accelerate cell proliferation substantially by speeding progression through G1/S as well as mitotic exit.

References

Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
Devroe E. et al., 2004 J Biol Chem. 279 (23): 24444-51.
Praskova M. et al., 2008, Curr Biol. 18 (5): 311-21.

MOB1B (MOBKL1A) related areas, pathways, and other information