MFAP5 / MAGP2 Protein (His Tag)

23.11.2012

Sino Biological Inc. liefert MFAP5 / MAGP2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über MFAP5 / MAGP2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/MFAP5-MAGP2-Protein-g-9908.html

Produkt-Information von MFAP5 / MAGP2 Protein

Synonym	MFAP5
Protein Construction	A DNA sequence encoding the human MFAP5 (Q13361) (Met1-Leu173) with a C-terminal polyhistidine tag was expressed.
Source	Human
Expression Host	Human Cells

QC Testing von MFAP5 / MAGP2 Protein

Purity	>(54.7+31.5)% as determined by SDS-PAGE	SDS-PAGE: MFAP5 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Arg 22
Molecular Mass	The recombinant human MFAP5 comprises 163 amino acids and has a predicted molecular mass of 18.7 kDa. The apparent molecular mass of the protein is approximately 54 and 29 kDa in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von MFAP5 / MAGP2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von MFAP5 / MAGP2 Protein

MFAP5, also called MAGP2, is a secreted, cell-associated 25 kDa mammalian member of the MFAP family of proteins. MFAP5 is rich in serine and threonine residues. It also has multiple binding regions on fibrillins and has covalent periodic association with fibrillin-containing microfibrils. As a 25-kD microfibril-associated glycoprotein, MFAP5 stimulates the assembly of elastic fibers, a complex structure composed of a tropoelastin inner core and microfibril outer mantle comprising proteins such as fibrillins and microfibril-associated glycoproteins that guide tropoelastin deposition. MFAP5 can function also outside of its role in elastic fibers and activates cellular signaling pathways. It interacts with the EGF-like repeats of Notch-1, which leads to both cell surface release of the Notch-1 extracellular domain and subsequent activation of Notch signaling.

References

Hatzinikolas G. et al., 1998, J Biol Chem. 273 (45): 29309-14.
Gibson MA. et al., 1996, J Biol Chem. 271 (2): 1096-103.
Miyamoto A. et al., 2006, J Biol Chem. 281 (15):10089-97.