B3GNT2 Protein

23.11.2012

Sino Biological Inc. liefert B3GNT2 Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über B3GNT2 Protein lesen Sie bitte: http://www.sinobiological.com/B3GNT2-Protein-g-9907.html

Produkt-Information von B3GNT2 Protein

Synonym	B3GNT2, B3GALT7, B3GNT1
Protein Construction	A DNA sequence encoding the human B3GNT2 (Q9NY97-1)(Lys29-Cys397) was expressed ,with the fused Fc region of human IgG1 at the N-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von B3GNT2 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: B3GNT2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Glu
Molecular Mass	The recombinant human B3GNT2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 629 amino acids and has a predicted molecular mass of 71.2 kDa. The apparent molecular mass of the protein is approximately 112-120 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von B3GNT2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von B3GNT2 Protein

B3GNT2 is a member of the beta-1,3-N-acetylglucosaminyltransferase family. As a type II transmembrane protein, it prefers the substrate of lacto-N-neotetraose. Alternative splicing produced 2 isoforms of the human protein. Enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. B3GNT2 catalyzes the initiation and elongation of poly-N- acetyllactosamine chains. B3GNT2 and beta3Gn-T8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone. It is found that up-regulation of beta3Gn-T8 in differentiated HL-60 cells may increases poly-N-acetyllactosamine chains by activating intrinsic B3GNT2.

References

Australo-Anvc .et al., 2010, Nat Genet. 42 (2): 123-7.
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
Seko A. et al., 2008, J Biol Chem. 283 (48): 33094-100.