CMPK1 Protein (His Tag)

10.01.2013

Sino Biological Inc. liefert CMPK1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CMPK1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CMPK1-Protein-g-10321.html

Produkt-Information von CMPK1 Protein

Synonym	CMPK1, CMK, CMPK, UCK, UMK, UMPK
Protein Construction	A DNA sequence encoding the human CMPK1 (P30085)(Met1-Gly196) was fused with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von CMPK1 Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: CMPK1 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human CMPK1 consists of 214 amino acids and has a calculated molecular mass of 24.5 kDa. The recombinant protein migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 7.4,10% glycerol. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CMPK1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CMPK1 Protein

CMPK1 plays a key role in the maintenance of pyrimidine nucleotide pool profile and for the metabolism of pyrimidine analogs in cells. It catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. CMPK1 also has a significant role in the activation of pyrimidine analogs, which are clinically useful anti-cancer and anti-viral drugs. In the meanwhile, CMPK1 functions in cellular nucleic acid biosynthesis.

References

Liou J. et al., 2004, Molecular Pharmacology. 67 (3): 806-14.
Gerhard DS. et al., 2004, Genome Research. 14 (10B): 2121-7.
Segura-Pena D. et al., 2004, Journal of Biological Chemistry. 279 (32): 33882-9.