ING5 Protein (GST Tag)

22.10.2012

Produkt-Information von ING5 Protein

Synonym	ING5
Protein Construction	A DNA sequence encoding the mature form of human ING5(Q8WYH8-2) (Glu36-Gln84) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von ING5 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: ING5 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human ING5 /GST chimera consists of 283 amino acids and has a predicted molecular mass of 32.9 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ING5 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ING5 Protein

ING5 is a member of the ING family. It is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. ING5 contains 1 PHD-type zinc finger. It can bind TP EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. ING5 is a component of the HBO1 complex. HBO1 complex has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of PHF15, PHF16 and PHF17. ING5 also is a component of the MOZ/MORF complex which is composed at least of ING5, KAT6A, KAT6B, MEAF6 and one of BRPF1, BRD1/BRPF2 and BRPF3. It interacts with EP300 and TP53. MOZ/MORF complex has a histone H3 acetyltransferase activity. Through chromatin acetylation ING5 may regulate DNA replication and may function as a transcriptional coactivator. It interacts with H3K4me3 and to a lesser extent with H3K4me2.

References

Zhang F. et al., 2011, PLoS One. (7): e21505.
Zheng HC. et al., 2011, Hum Pathol. 42 (3): 424-33.
Xing YN. et al., 2011, Hum Pathol. 42 (1): 25-35.

Sino Biological Inc. liefert ING5 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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