Influenza A H9N2(A/Chicken/Hong Kong/G9/97)HA Protein (His Tag)

22.10.2012

Produkt-Information von H9N2 (A/Chicken/Hong Kong/G9/97) HA Protein

Synonym	HA
Protein Construction	A DNA sequence encoding the N-terminal segment (Met1-Lys523) of the influenza hemagglutin (Influenza A virus (A/Chicken/Hong Kong/G9/97(H9N2))(AAF00701.1), termed as HA, was fused with a polyhistidine tag at the C-terminus.
Source	Influenza A Virus
Expression Host	Human Cells

QC Testing von H9N2 (A/Chicken/Hong Kong/G9/97) HA Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: H9N2 HA protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Asp 19
Molecular Mass	The secreted recombinant HA1 subunit of influenza A H9N2 HA(Influenza A virus (A/Chicken/Hong Kong/G9/97(H9N2)) comprises 516amino acids and has a predicted molecular mass of 58.2 kDa. As a result of glycosylation, it migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von H9N2 (A/Chicken/Hong Kong/G9/97) HA Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von H9N2 (A/Chicken/Hong Kong/G9/97) HA Protein

Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.

References

Barman S. et al., 2000, J Virol. 74: 6538-45.
Suzuki T. et al., 2005, J Virol. 79: 11705-15.
Shinya K. et al., 2006, Nature. 440 (7083): 435-6.
Marjuki H. et al., 2006, J Biol Chem. 281: 16707-15.
Christophe F. et al., 2009, Science. 324: 1557-61.
Von Itzstein M. 2007, Nat Rev Drug Discov. 6: 967-74.

Sino Biological Inc. liefert Influenza A H9N2 HA Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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