IL6RA / CD126 ELISA Pair Set, Mouse ELISA Pair Set

Fängerantikörper 0.5 mg/mL of rabbit anti-mouse IL6RA monoclonal antibody, Dilute to a working concentration of 1 μg/mL in CBS before coating.

Nachweisantikörper 0.25 mg/mL rabbit anti-mouse IL6RA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 20 ng of recombinant mouse IL6RA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.

The minimum detectable dose of Mouse IL6RA / CD126 was determined to be approximately 6.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Mouse IL6RA / CD126 ELISA Pair Set is for the quantitative determination of Mouse IL6RA / CD126.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL6RA / CD126 coated on a 96-well plate. Standards and samples are added to the wells, and any IL6RA / CD126 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-IL6RA / CD126 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IL6RA / CD126 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Interleukin 6 receptor subunit alpha, also known as IL6R1, CD126, and IL6Ra, is a type I transmembrane cytokine receptor and lacks a tyrosine/kinase domain within the cytoplasmic region, unlike other growth factor receptors. The IL6 receptor is composed of two different subunits, IL6R that binds IL6 with low affinity, and ubiquitously expressed Glycoprotein 130 (gp130) which acts as a common signal-transducing receptor and binds the IL-6·IL-6R complex with high affinity. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis. It has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases.