CD157 / BST1 ELISA Pair Set, Mouse ELISA Pair Set

Fängerantikörper 1.0 mg/mL of rabbit anti-mouse BST1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL rabbit anti-mouse BST1 polyoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 65 ng of recombinant mouse BST1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.

The minimum detectable dose of Mouse CD157 / BST1 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Mouse CD157 / BST1 ELISA Pair Set is for the quantitative determination of Mouse CD157 / BST1.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD157 / BST1 coated on a 96-well plate. Standards and samples are added to the wells, and any CD157 / BST1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD157 / BST1 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD157 / BST1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

CD157, also known as bone marrow sromal cell antigen 1 (BST-1), is a glycosylphosphatidylinositol-anchored ectoenzyme belonging to the NADase/ADP-ribosyl cyclase family. It is expressed in the bone marrow, spleen and thymus in lymphoid organs, and the lung, kidney and heart in non-lymphoid organs. In mouse, CD157 is also expressed on lymphoid progenitors. It has a dual nature behaving both as an ectoenzyme and as a receptor. Cross-linking of CD157 induces intracellular Ca2+ mobilization and re-shaping in neutrophils, thus regulating their adhesive and migratory properties. The CD157-mediated signals promote cell polarization, regulate chemotaxis induced through the high affinity formyl-methionyl-leucyl-phenylalanine (fMLP) receptor and control transendothelial migration. CD157 exhibits homology with CD38 and Aplysia ADP-ribosyl cyclase. Like CD38, it has both ADP-ribosyl cyclase and cADPR hydrolase activities. The CD157 gene is synthenic on 4p15 with CD38, with which it also shares a unique genomic organization. Their conservation in phylogeny is striking evidence for their relevance in the life and death cycle of the cell. CD157 has a specific and crucial role in the regulation of innate immunity during inflammation. The murine CD157 is a useful marker for lymphoid progenitor cells initiating gene rearrangement of their antigen receptors.