OSMR / IL-31RB ELISA Pair Set, Human ELISA Pair Set

Fängerantikörper 0.5 mg/mL of rabbit anti-OSMR monoclonal antibody, Dilute to a working concentration of 1 μg/mL in CBS before coating.

Nachweisantikörper 0.5 mg/mL mouse anti-OSMR monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody diluteion buffer before use.

Standard Each vial contains 600 ng of recombinant OSMR. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 15,000 pg/mL is recommended.

The minimum detectable dose of Human OSMR / IL-31RB was determined to be approximately 234.4 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

The Human OSMR / IL-31RB ELISA Pair Set is for the quantitative determination of Human OSMR / IL-31RB.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for OSMR / IL-31RB coated on a 96-well plate. Standards and samples are added to the wells, and any OSMR / IL-31RB present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-OSMR / IL-31RB monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of OSMR / IL-31RB present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

Speicherung - Detection Antibody should be protected from prolonged exposure to light. Aliquot the reagents and store at -20℃ to -70℃ in a manual defrost freezer.

Oncostatin-M-specific receptor subunit beta, also known as interleukin-31 receptor subunit beta, and OSMR, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 2 subfamily. OSMR contains four fibronectin type-III domains and is expressed at relatively high levels in all neural cells as well as fibroblast, epithelial and a variety of tumor cell lines. Four forms of OSMR have been identified: leukemia inhibitory factor receptor (LIFR), OSMR beta, short-form OSMR (OSMRs) and soluble OSMR (sOSMR). OSMR associates with IL31RA to form the IL31 receptor. It binds IL31 to activate STAT3 and possibly STAT1 and STAT5. OSMR is part of a heterodimeric receptor complex that mediates signal transduction of the pleiotropic cytokine OSM via a signaling pathway involving Janus kinases (Jaks) and transcription factors of the signal transducers and activators of transcription (STAT) family. Defects in OSMR are the cause of amyloidosis type 9 (AMYL9) which is a hereditary primary amyloidosis characterized by localized cutaneous amyloid deposition.