IL-1 alpha / IL-1F1 / IL1A Antikörper (Antigen Affinität reinigt)

03.07.2012

Produkt-Information von IL-1 alpha / IL-1F1 / IL1A Antikörper

Immunogen	Recombinant Mouse IL-1 alpha / IL-1F1 / IL1A protein (Catalog#50114-MNAE)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Mouse IL-1 alpha / IL-1F1 / IL1A (rM IL-1 alpha / IL-1F1 / IL1A; Catalog#50114-MNAE; NP_034684.2; Ser 115-Ser 270). IL-1 alpha / IL-1F1 / IL1A specific IgG was purified by Mouse IL-1 alpha / IL-1F1 / IL1A affinity chromatography.

Usage Guide von IL-1 alpha / IL-1F1 / IL1A Antikörper

Specificity

Mouse IL-1 alpha / IL-1F1 / IL1A

Immunochemistry

IHC-P: 0.5-3 μg/mL

mIL1A-mouse-brain immunohistochemistry

Immunochemical staining of mouse IL1A in mouse-brain with rabbit polyclonal antibody (2 µg/mL, formalin-fixed paraffin embedded sections).
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

Western blot

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse IL1A in WB. Using a DAB detection system, the detection limit for Mouse IL1A is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hintergrund von IL-1 alpha / IL-1F1 / IL1A Antikörper

Interleukin 1 (IL1) is an important cytokine mediator involved in a variety of biological processes including immune responses, inflammatory, and promoting tissue degradation. IL1 comprises two proteins, designated as IL-1 alpha/IL1α and IL-1 beta/IL1β which are encoded by distinct genes, and bind the same surface receptors with high affinity. IL1 proteins are synthesized as 31 kDa cytosolic precursors in monocytes and macrophages without signal peptides, and are proteolytically processed and released as 17kDa mature molecules in response to various stimulus not via the classical secretory pathway. It has been reported that release of IL1 is efficiently induced by cell injury. IL-1 alpha/L1α is released as a mixture of unprocessed and processed molecules cleaved by a calpain-like protease, and both of the forms show full biological activity. Elevated levels of IL-1 alpha/IL1α have been associated with a number of chronic disorders, including rheumatoid arthritis and Alzheimer's disease, and therefore, may provide novel targets for therapeutic approaches aimed at controlling inflammatory processes.

References

Dinarello, C. A. et al., 1996, Blood. 87: 2095-2147.
Hogguist, K.A. et al., 1991, Proc. Natl. Acad. Sci. 88: 8485-8489.
Griffin, W.S. et al. 2002, J. Leukoc. Biol. 72: 233-238.
Perreqaux, D.G. et al., 1998, J. Immunol. 160: 2469-2477.
Brunn, G.J. et al., 2008, Circ. Res. 102: 823-830.

Sino Biological Inc. liefert IL-1 alpha / IL-1F1 / IL1A Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über IL-1 alpha / IL-1F1 / IL1A Antikörper (Antigen Affinität reinigt) lesen Sie bitte: http://www.sinobiological.com/IL-1-alpha-IL-1F1-IL1A-Antibody-g-9160.html

IL-1 alpha / IL-1F1 / IL1A Antikörper (Antigen Affinität reinigt)

Produkt-Information von IL-1 alpha / IL-1F1 / IL1A Antikörper

Usage Guide von IL-1 alpha / IL-1F1 / IL1A Antikörper

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Hintergrund von IL-1 alpha / IL-1F1 / IL1A Antikörper

References