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Produkt-Information von IkB alpha / NFKBIA Antikörper
Immunogen
Recombinant Human IkB alpha / NFKBIA Protein (Catalog#12045-H07E)
Reagents
FITC-conjugated mouse monoclonal antibody
Clone ID
4H9G5
Ig Type
Concentration
5 μl/Test, 0.2 mg/ml
Formulation
Aqueous solution containing 0.5% BSA and 0.1% sodium azide
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IkB alpha / NFKBIA (rh IkB alpha / NFKBIA; Catalog#12045-H07E; NP_065390.1; Phe 2-Leu 317) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
IkB alpha / NFKBIA Antikörper Usage Guide
Specificity
Human IkB alpha / NFKBIA
Flow Cytometry
Fig 1, Fig 2. NFKBIA expression in HeLa and Jurkat cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Mouse anti-NFKBIA. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Fig 1. NFKBIA expression in HeLa cells
Fig 2. NFKBIA expression in Jurkat cells
Flow cytometry was performed on a BD FACSCalibur flow cytometry system
This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NFKBIA in WB.
Storage
This antibody is stable for 24 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Verwandte Produkte & Themen von IkB alpha / NFKBIA Antikörper
Verwandte Bereichen von IkB alpha / NFKBIA Antikörper:
Proteins:
Antibodies:
Hintergrund von IkB alpha / NFKBIA Antikörper
NF-kappa-B inhibitor alpha, also known as Major histocompatibility complex enhancer-binding protein MAD3, I-kappa-B-alpha, NFKBIA and IKBA, is a cytoplasm and nucleus protein which belongs to theNF-kappa-B inhibitor family. NFKBIA contains five ANK repeats. NFKBIA inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, NFKBIA becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.
References
Scherer D.C. et al.,1995, Proc. Natl. Acad. Sci. USA. 92: 11259-11263.
Jungnickel B. et al., 2000, J. Exp. Med. 191: 395-402.
Courtois G. et al., 2003, J. Clin. Invest. 112: 1108-1115.
Ota T. et al., 2004, Nat. Genet. 36: 40-45.
Sino Biological Inc. liefert IkB alpha / NFKBIA Antikörper mit höherer Qualität und günstigen Kosten im Vergleich mit anderen globalen Lieferanten.
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