IkB alpha / NFKBIA Antikörper

21.05.2012

Produkt-Information von IkB alpha / NFKBIA Antikörper

Immunogen	Recombinant Human IkB alpha / NFKBIA protein (Catalog#12045-H07E)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	4H9G5
Ig Type
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IkB alpha / NFKBIA (rh IkB alpha / NFKBIA; Catalog#12045-H07E; NP_065390.1; Phe 2-Leu 317). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von IkB alpha / NFKBIA Antikörper

Specificity

Human IkB alpha / NFKBIA

Immunochemistry

ICC/IF: 5-20 μg/mL

NFKBIA-MM02-15ug-HeLa-IF Immunofluorescence

Immunofluorescence staining of human NFKBIA in HeLa cells with mouse monoclonal antibody (15 μg/mL). The image showing nucleus staining of HeLa cells.

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human NFKBIA in WB.

Direct ELISA

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human NFKBIA.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von IkB alpha / NFKBIA Antikörper

Related Areas:

Signal Transduction>>NFkB pathway>>IkB alpha/NFKBIA

Cancer>>NFkB pathway>>IkB alpha/NFKBIA

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
IkB alpha/NFKBIA	Human	IkB alpha/NFKBIA Protein, Recombinant	12045-H07E

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human IkB alpha / NFKBIA	WB, ELISA, ICC/IF IF	IkB alpha / NFKBIA Antibody	12045-MM02
Human IkB alpha / NFKBIA	WB, ELISA, ICC/IF	IkB alpha / NFKBIA Antibody, Mouse Mab	12045-MM02-F
Human IkB alpha / NFKBIA	WB, ELISA	IkB alpha / NFKBIA Antibody, Mouse Mab	12045-MM03
Human IkB alpha / NFKBIA	WB, ELISA	IkB alpha / NFKBIA Antibody (Antigen Affinity Purified)	12045-RP02
Human IkB alpha / NFKBIA	WB, ELISA	IkB alpha / NFKBIA Antibody	12045-R116

Hintergrund von IkB alpha / NFKBIA Antikörper

NF-kappa-B inhibitor alpha, also known as Major histocompatibility complex enhancer-binding protein MAD3, I-kappa-B-alpha, NFKBIA and IKBA, is a cytoplasm and nucleus protein which belongs to theNF-kappa-B inhibitor family. NFKBIA contains five ANK repeats. NFKBIA inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, NFKBIA becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. Defects in NFKBIA are the cause of ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant (ADEDAID). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ADEDAID is an ectodermal dysplasia associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.

References

Scherer D.C. et al.,1995, Proc. Natl. Acad. Sci. USA. 92: 11259-11263.
Jungnickel B. et al., 2000, J. Exp. Med. 191: 395-402.
Courtois G. et al., 2003, J. Clin. Invest. 112: 1108-1115.
Ota T. et al., 2004, Nat. Genet. 36: 40-45.

Sino Biological Inc. liefert IkB alpha / NFKBIA Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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