HPGD / 15-PGDH Antikörper

21.05.2012

Produkt-Information von HPGD / 15-PGDH Antikörper

Immunogen	Recombinant Human HPGD / 15-PGDH protein (Catalog#11205-H08E)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	2B10G12D2
Ig Type
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HPGD / 15-PGDH (rh HPGD / 15-PGDH; Catalog#11205-H08E; NP_000851.2; Met 1-Gln 266). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von HPGD / 15-PGDH Antikörper

Specificity

Human HPGD / 15-PGDH

Immunochemistry

IHC-P: 15-30 μg/mL

HPGD-bladder-carcinoma immunohistochemistry

Immunochemical staining of human HPGD in human bladder carcinoma with mouse monoclonal antibody (25 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to transitional epithelium.

HPGD-liver immunohistochemistry

Immunochemical staining of human HPGD in human liver with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections).
The left panel tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human HPGD in WB.

Direct ELISA

This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect Human HPGD.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hintergrund von HPGD / 15-PGDH Antikörper

15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor inmany species and regulate uterine contractility and cervicaldilatation. Therefore, the regulation of prostaglandin outputby PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activitypatterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR), cranioosteoarthropathy (COA),and isolated congenital nail clubbing.

References

Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33.
McKeown KJ, et al.,2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41.
Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73.
Tariq, M. et al., 2009,J Med Genet. 46 (1): 14-20.

Sino Biological Inc. liefert HPGD / 15-PGDH Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über HPGD / 15-PGDH Antikörper lesen Sie bitte: http://www.sinobiological.com/HPGD-15-PGDH-Antibody-g-8859.html

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
HPGD/15-PGDH	Human	HPGD/15-PGDH Protein, Recombinant	11205-H08E
HPGD/15-PGDH	Mouse	HPGD/15-PGDH Protein, Recombinant	50531-M08E

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human HPGD/15-PGDH	WB, ELISA	Mouse Monoclonal Antibody	11205-MM01
Human HPGD/15-PGDH	WB, ELISA, IHC-P	HPGD / 15-PGDH Antibody	11205-MM02
Mouse HPGD/15-PGDH	WB, ELISA	HPGD / 15-PGDH Antibody	50531-RP01
Mouse HPGD/15-PGDH	WB, ELISA	HPGD / 15-PGDH Antibody (Antigen Affinity Purified)	50531-RP02

HPGD / 15-PGDH Antikörper

Produkt-Information von HPGD / 15-PGDH Antikörper

Usage Guide von HPGD / 15-PGDH Antikörper

Verwandte Produkte & Themen von HPGD / 15-PGDH Antikörper

Hintergrund von HPGD / 15-PGDH Antikörper

References