HMGN3 Protein (His Tag)

17.10.2012

Produkt-Information von HMGN3 Protein

Synonym	HMGN3, TRIP7
Protein Construction	A DNA sequence encoding the mature form of human HMGN3(Q15651-2) (Met1- Asn77) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von HMGN3 Protein

Purity	> 96% as determined by SDS-PAGE	SDS-PAGE: HMGN3 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human HMGN3 consists of 92 amino acids and predicts a molecular mass of 10.2 KDa. It migrates as an approximately 16 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 50mM Tris 50mM Arg 10% glycerol pH 8.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von HMGN3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von HMGN3 Protein

HMGN3 is a member of the HMGN family. It can be detected in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. It interacts with nucleosomes and its interaction requires the presence of thyroid hormone. In vitro, HMGN3 interacts with the ligand binding domain of the thyroid receptor (TR). HMGN3 regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. It also regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. It binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Both insulin and glucagon levels can be affected by HMGN3. HMGN3 may play a role in ocular development and astrocyte function. It also modulates the expression of pancreatic genes involved in insulin secretion.

References

Ueda T. et al., 2009, Mol Cell Biol. 29 (19): 5264-76.
Lei SF. et al., 2012, Hum Genet. 131 (3): 463-9.
Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.

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