ABHD14B Protein (His Tag)

17.10.2012

Produkt-Information von ABHD14B Protein

Synonym	ABHD14B, CIB
Protein Construction	A DNA sequence encoding the mature form of human ABHD14B (Q96IU4-1) (Met1-Gln210) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von ABHD14B Protein

Purity	> 96% as determined by SDS-PAGE	SDS-PAGE: ABHD14B protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human ABHD14B consists of 225 amino acids and predicts a molecular mass of 24.2 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of PBS 20% glycerol 50mM Arg 0.1%Tween20 pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ABHD14B Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ABHD14B Protein

ABHD14B is a member of the AB hydrolase superfamily, ABHD14 family. It contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members. ABHD14B can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

References

Mehrle A. et al., 2006, Nucleic Acids Res. 34: D415-8.
Wan D. et al., 2004, Proc Natl Acad Sci. 101 (44): 15724-9.
Wiemann S. et al., 2004, Genome Res. 14 (10B): 2136-44.

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