HMGB1 / HMG1 Protein (His Tag)

16.04.2013

Sino Biological Inc. liefert HMGB1 / HMG1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über HMGB1 / HMG1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/HMGB1-HMG1-Protein-a-630.html

Produkt-Information von HMGB1 / HMG1 Protein

Synonym :	HMGB1, HMG1
Protein Construction:	A DNA sequence encoding the mature form of human HMGB1 (P09429) (Lys57-Asp158) was expressed with a polyhistidine tag at the N-terminus.
Source:	Human
Expression Host:	E.coli

QC Testing von HMGB1 / HMG1 Protein

Purity:	> 90% as determined by SDS-PAGE	SDS-PAGE: HMGB1 protein
Endotoxin:	Please contact us for more information.
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	His
Molecular Mass:	The recombinant human HMGB1 consists of 117 amino acids and predicts a molecular mass of 13.6 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions.
Formulation:	Lyophilized from sterile PBS, 20% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von HMGB1 / HMG1 Protein

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von HMGB1 / HMG1 Protein

High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. HMGB1 is structurally composed of two homologous DNA-binding domains entitled box A and box B and a highly negative-charged C terminus. This protein is extremely conserved across species with 99-100% aa sequence homology to mouse, rat, bovine, canine, porcine proteins. As a nuclear protein, HMGB1 stabilizes nucleosomes and allows bending of DNA that facilitates gene transcription which is essential for individual survival. Meanwhile, it is revealed that HMGB1 can also act as a cytokine extracellularlly and regulates monocyte, T cell, dendritic cell activities in inflammatory responses. Once secreted, HMGB1 induces signal transduction through its receptor RAGE and perhaps TLR2 and TLR4. The immune-regulatory function of HMGB1 may lead to being identified as new therapeutic target.

References

Wen L. et al., 1989, Nucleic Acids Res. 17: 1197-214.
Ge H. et al., 1994, J BiolChem. 269: 17136-40.
Andersson,U. et al., 2002, J.Leukoc Biol. 1084-91.
Lotze MT. and K.J. Tracey, 2005, Nat Rev Immunol. 5: 331-42.
Degryse B. et al., 2003, FEBS Lett. 553: 11-17.
Kazama H. et al., 2008, Immunity. 29: 21-32.