HBP1 Protein (GST Tag)

06.03.2013

Sino Biological Inc. liefert HBP1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über HBP1 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/HBP1-Protein-g-10543.html

Produkt-Information von HBP1 Protein

Synonym	HBP1
Protein Construction	A DNA sequence encoding the mature form of human HBP1 (O60381-1) (Pro208-Phe345) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von HBP1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: HBP1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human HBP1 /GST chimera consists of 372 amino acids and has a predicted molecular mass of 42.5 kDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von HBP1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von HBP1 Protein

HBP1 is a sequence-specific DNA-binding transcription factor. It is involved in many biological processes. It was reported that HBP1 binds to p16(INK4A) promoter and activates p16(INK4A) expression. We found that trichostatin A (TSA), an inhibitor of HDAC (histone deacetylase), induces p16(INK4A) expression in an HBP1-dependent manner. HBP1 activates or represses the expression of some specific genes during cell growth and differentiation. HBP1 was acetylated by p300/CBP in two regions: repression domain (K297/305/307) and P domain (K171/419). HBP1 acetylation after TSA treatment was confirmed by immunoprecipitation assay. HBP1 interacted with histone acetyltransferase p300 and CREB-binding protein (CBP) and also recruited p300/CBP to p16(INK4A) promoter. HBP1 acetylation at K419 plays an important role in HBP1-induced p16(INK4A) expression.

References

Tevosian SG. et al., 1997, Genes Dev. 11 (3): 383-96.
Lavender P. et al., 1997, Oncogene. 14 (22): 2721-8.
Swanson. et al., 2004, Nat Struct Mol Biol. 11 (8): 738-46.