GUCA1A / GCAP 1 Protein (GST Tag)

25.04.2013

Sino Biological Inc. liefert GUCA1A / GCAP 1 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über GUCA1A / GCAP 1 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/GCAP1-GUCA1A-Protein-Antibody-a-5861.html

Produkt-Information von GUCA1A / GCAP 1 Protein

Synonym	GUCA1A, C6orf131, GCAP, GCAP1, GUCA1
Protein Construction	A DNA sequence encoding the human GUCA1A (P43080)( Met1-Gly201) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von GUCA1A / GCAP 1 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: GUCA1A protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant human GUCA1A consists of 438 amino acids and predicts a molecular mass of 50.7 KDa. The apparent molecular mass of the protein is approximately 42 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von GUCA1A / GCAP 1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GUCA1A / GCAP 1 Protein

GCAP 1 gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase activating protein 1 (GCAP 1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP 1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in GCAP 1 gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. GCAP 1 stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.

References

Surguchov A. et al., 1997, Genomics. 39 (3): 312-22.
Subbaraya I. et al., 1995, J Biol Chem. 269 (49): 31080-9.
Payne AM. et al., 1998, Hum Mol Genet. 7 (2): 273-7.

GCAP1 (GUCA1A) related areas, pathways, and other information