ECH1 Protein (His Tag)

25.04.2013

Sino Biological Inc. liefert ECH1 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ECH1 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/ECH1-Protein-Antibody-a-5870.html

Produkt-Information von ECH1 Protein

Synonym	ECH1
Protein Construction	A DNA sequence encoding the mature form of human ECH1 (Q13011) (Thr34-Leu328) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von ECH1 Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: ECH1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human ECH1 consists of 310 amino acids and predicts a molecular mass of 34 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ECH1 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ECH1 Protein

ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

References

Kovalyov LI. et al., 2006, Biochemistry Mosc. 71 (4): 448-53.
Olsen JV. et al., 2006, Cell. 127 (3): 635-48.
FitzPatrick DR. et al., 1995, Genomics. 27 (3): 457-66.

ECH1 related areas, pathways, and other information