ALOX15B Protein (GST Tag)

25.04.2013

Sino Biological Inc. liefert ALOX15B Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ALOX15B Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/ALOX15B-15-Lipoxygenase-2-Protein-Antibody-a-5862.html

Produkt-Information von ALOX15B Protein

Synonym	ALOX15B
Protein Construction	A DNA sequence encoding the human ALOX15B (AAH35217.1)( Met1-Ile676) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von ALOX15B Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: ALOX15B protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The secreted recombinant human ALOX15B consists of 913 amino acids and predicts a molecular mass of 103.7 KDa. The apparent molecular mass of the protein is approximately 93 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ALOX15B Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ALOX15B Protein

ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. ALOX15B converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. ALOX15B gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.

References

Kilty I. et al., 2000, Eur J Biochem. 266 (1): 83-93.
Sigal E. et al., 1990, J Biol Chem. 265 (9): 5113-20.
Brash AR. et al., 1997, Proc Natl Acad Sci. 94 (12): 6148-52.

ALOX15B (15 Lipoxygenase 2) related areas, pathways, and other information