GPR37 Protein (His Tag)

29.03.2012

Produkt-Information von GPR37 Protein

Synonym	EDNRBL, PAELR, hET(B)R-LP
Protein Construction	A DNA sequence encoding the human GPR37 (P17643) the first extracellular domaqin (Met 1- Met 265) was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von GPR37 Protein

Purity	> 80 % as determined by SDS-PAGE	SDS-PAGE: GPR37 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 27
Molecular Mass	The secreted recombinant human GPR37 comprises 250 amino acids and has a predicted molecular mass of 26.6 kDa. ? The apparent molecular mass of rh GPR37 is approximately 30-45 kDa in SDS-PAGE under reducing conditions due to different glycosylation.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

GPR37 Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GPR37 Protein

GPR37(cathepsin Z, Histidine-tagged) is an orphan receptor which belongs to the G-protein coupled receptor 1 family. It is an orphan receptor and may have a unique functional role in the central nervous system. GPR37, expressed at highest levels in human brain,spinal cord and at lower levels in testis, placenta, liver, with homology to bombesin receptor. When overexpressed in cells, tends to become insoluble and unfolded. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease. GPR37 forms a complex with PARK2, STUB1 and HSP70. The amount of STUB1 in the complex increases during ER stress. STUB1 promotes the dissociation of HSP70 from PARK2, thus facilitating PARK2-mediated GPR37 ubiquitination. It interacts with PACRG and is ubiquitinated by PARK2 in the presence of UBE2E1 and UBE2L3 in the endoplasmic reticulum. The unfolded form is specifically ubiquitinated by SYVN1, which promotes its proteasomal degradation and prevents neuronal cell death.

References

Marazziti D. et al., 1997, Genomics. 45 (1): 68-77.
Imai. et al., 2002, Mol Cell (United States). 10 (1): 55-67.
Imai. et al., 2001, Cell (United States). 105.(7): 891-902.

Sino Biological Inc. liefert GPR37 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über GPR37 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/GPR37-Protein-g-8644.html