DCX (aa 45-150) Protein (GST Tag)

29.03.2012

Produkt-Information von DCX Protein

Synonym	RP5-914P14.1, DBCN, DC, LISX, SCLH, XLIS
Protein Construction	A DNA sequence encoding the human DCX (O43602-2) N-terminal fragment (Ala 45-Val 150) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von DCX Protein

Purity	> 82 % as determined by SDS-PAGE	SDS-PAGE: DCX protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human DCX (aa 45-150)/GST chimera consists of 340 amino acids and has a predicted molecular mass of 39.4 kDa. It migrates as an approxiamtely 36 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,1mM DTT,10% glycerol;pH7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

DCX Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von DCX Protein

DCX(doublecortin,N-GST chimera) belongs to the doublecortin family. It is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, it interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. DCX seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. It may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It also may be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.

References

Des Portes V. et al., 1998. Cell. 92:51-61.
Gleeson J G. et al., 1998. Cell. 92: 63-72.
Ross MT. et al., 2005. Nature. 434: 325-37.

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