GOLM1 / GP73 Antikörper

Produkt-Information von GOLM1 / GP73 Antikörper

Usage Guide von GOLM1 / GP73 Antikörper

Specificity	Human GOLM1 / GP73
Flow Cytometry	Flow cytometric analysis of Human GOLM1(GP73) expression in HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Rabbit anti-GOLM1(GP73) (13066-R024-F). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCalibur flow cytometry system Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human GOLM1 in WB. Using a DAB detection system, the detection limit for Human GOLM1 is approximately 1 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.
Storage	This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

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Hintergrund von GOLM1 / GP73 Antikörper

Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to theGOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.

References

1. Kladney R.D., et al., 2000,Gene 249:53-65.
2. Kladney R.D., et al., 2002, Virology 301:236-246.
3. Ramachandran P., et al., 2006, J. Proteome Res. 5:1493-1503.
4. Zhou W.,et al., 2009, J. Proteome Res. 8:5523-5531.

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