GNG13 Protein (His Tag)

07.05.2012

Produkt-Information von GNG13 Protein

Synonym	G(gamma)13, h2-35
Protein Construction	A DNA sequence encoding the mature form of human GNG13 (Q9P2W3) (Met 1-Cys 64) was expressed, with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von GNG13 Protein

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: GNG13 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human GNG13 consists of 80 amino acids and has a calculated molecular mass of 9.7kDa. It migrates as an approximately 9& 18KDa doublet in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von GNG13 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GNG13 Protein

GNG13 is a subunit of heterotrimeric G proteins. Heterotrimeric G proteins is composed of alpha, beta, and gamma subunits. Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. They function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. They are involved as a modulator or transducer in various transmembrane signaling systems. GNG13 is a gamma subunit and plays a key role in taste transduction. It is expressed in taste, retinal, and neuronal tissues. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; in addition, some Gbetagamma subunits have active functions. Gbetagamma coupled to H1 receptors can activate PLA2 and Gbetagamma coupled to M1 receptors can activate KIR channels. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

References

Huang L. et al., 2000, Nat Neurosci. 2 (12): 1055-62.
Blake B L. et al., 2001, J Biol Chem. 276 (52): 49267-74.
Bonaldo MF. et al., 1997, 6 (9): 791-806.

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