PGDH / PHGDH Protein (His Tag)

07.05.2012

Produkt-Information von PGDH / PHGDH Protein

Synonym	RP4-683H9.1, 3-PGDH, 3PGDH, PDG, PGAD, PGD, PGDH, SERA
Protein Construction	A DNA sequence encoding the mature form of human PHGDH (O43175) (Met 1-Phe 533) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von PGDH / PHGDH Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: PGDH / PHGDH protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human PHGDH comprises 543 amino acids and has a calculated molecular mass of 58KDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS,100mM Arg, 0.1% Tween20, 20%glycerol; pH8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PGDH / PHGDH Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PGDH / PHGDH Protein

PHGDH belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family which is comprised by D-isomer-stereospecific enzymes. It seems that the conserved residues in the D-isomer specific 2-hydroxyacid dehydrogenase family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

References

Pind S. et al., 2002, J Biol Chem. 277 (9): 7136-43.
Du H. et al., 2010, Reproduction. 139 (1): 237-45.
Possemato R. et al., 2011, Nature. 476 (7360): 346-50.

Sino Biological Inc. liefert PGDH / PHGDH Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über PGDH / PHGDH Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/PGDH-PHGDH-Protein-g-9024.html