PSPH Protein (Native)

18.04.2012

Produkt-Information von PSPH Protein

Synonym	PSP, PSPHD
Protein Construction	A DNA sequence encoding the human PSPH (P78330) (Met 1-Glu 225) was expressed and purified.
Source	Human
Expression Host	E.coli

QC Testing von PSPH Protein

Purity	> 84 % as determined by SDS-PAGE	SDS-PAGE: PSPH protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met 1
Molecular Mass	The recombinant human PSPH consists of 225 amino acids and has a calculated molecular mass of 25KD. It migrates as an approximately 28KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von PSPH Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von PSPH Protein

Phosphoserine phosphatase (PSPH) belongs to a subfamily of the phosphotransferases and is the rate-limiting enzyme in l-serine biosynthesis. It has previously been found that Phosphoserine phosphatase (PSPH) expression to be uniquely upregulated in a α6β4 integrin transgenic mouse model that is predisposed to epidermal hyperproliferation and squamous cell carcinoma (SCC) formation implicating a role for Psph in epidermal homeostasis. Phosphoserine phosphatase (PSP) catalyzes the hydrolysis of phosphoserine to serine. Phosphoserine phosphatase (PSPH) expression has been examined in human-mouse somatic cell hybrids retaining different combination of human chromosomes. Human Phosphoserine phosphatase (PSPH) is expressed only when the pter→q22 segment of the human 7 and its enzyme marker qβ-glucuronidase (GUSB) are retained in cell hybrids. Phosphoserine phosphatase (PSPH) is expressed throughout the proliferative layer of the epidermis and hair follicles in rodent and human skin and is highly induced in SCC. In keratinocytes, Phosphoserine phosphatase (PSPH) is a cytoplasmic protein that primarily localizes to endosomes and is present primarily as a homodimer. Knock down of Phosphoserine phosphatase (PSPH) dramatically diminished SCC cell proliferation and cyclin D1 levels in the presence of exogenous of l-serine production suggesting a non-canonical role for Phosphoserine phosphatase (PSPH) in epithelial carcinogenesis. Phosphoserine phosphatase (PSPH) is highly induced in proliferative normal keratinocytes and in skin tumors. Phosphoserine phosphatase (PSPH) appears to be critical for the proliferation of SCC cells; however, this phenomenon may not involve the phosphoserine metabolic pathway.

References

Bachelor MA. et al., 2011, J Dermatol Sci. 63 (3): 164-72.
Koch GA. et al., 1983, Cytogenet Cell Genet. 35 (1): 67-9.
Jaeken J. et al., 1997, J Med Genet. 34 (7): 594-6.

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