GCSH Protein (His Tag)

09.05.2013

Sino Biological Inc. liefert GCSH Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über GCSH Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/GCSH-Protein-Antibody-a-5912.html

Produkt-Information von GCSH Protein

Synonym	GCSH
Protein Construction	A DNA sequence encoding the mature form of human GCSH (AAA36011.1) (Ser49-Glu173) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von GCSH Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: GCSH protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human GCSH consists of 140 amino acids and predicts a molecular mass of 15.7 KDa. It migrates as an approximately 17 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von GCSH Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

GCSH Protein Description

Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in GCSH gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding,have been found for GCSH gene. Also, several transcribed and non-transcribed pseudogenes of GCSH gene exist throughout the genome.

References

Hiraga K. et al., 1988, Biochem Biophys Res Commun. 151 (2): 758-62.
Fujiwara K. et al., 1991, Biochem Biophys Res Commun. 176 (2): 711-6.
Koyata H. et al., 1991, Am J Hum Genet. 48 (2): 351-61.