CBFB Protein (His Tag)

09.05.2013

Sino Biological Inc. liefert CBFB Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CBFB Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CBFB-CBF-beta-Protein-Antibody-a-5915.html

Produkt-Information von CBFB Protein

Synonym	CBFB
Protein Construction	A DNA sequence encoding the mature form of human CBFB (Q13951-1) (Met1-Pro182) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von CBFB Protein

Purity	> 85 % as determined by SDS-PAGE	SDS-PAGE: CBFB protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human CBFB consists of 197 amino acids and predicts a molecular mass of 23.3 KDa. It migrates as an approximately 27 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CBFB Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CBFB Protein

CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). CBFB is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for CBFB gene. Mutations in CBFB are implicated in cases of breast cancer.

References

Hart SM. et al., 2003, Haematologica. 87(12): 1307-23.
Liu P. et al., 1995, Cold Spring Harb Symp Quant Biol. 59: 547-53.
Liu P. et al., 1993, Science. 261 (5124): 1041-4.