EPHB2 / Hek5 (aa 570-987) Protein (GST Tag)

Sino Biological Inc. liefert EPHB2 / Hek5 (aa 570-987) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über EPHB2 / Hek5 (aa 570-987) Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/EPHB2-Hek5-Protein-g-9867.html

Produkt-Information von EPHB2 / Hek5 Protein

QC Testing von EPHB2 / Hek5 Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: EPHB2 / Hek5 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human EPHB2 /GST chimera consists of  655 amino acids and has a calculated molecular mass of 75.2 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 3mM DTT, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von EPHB2 / Hek5 Protein

Verwandte Produkte & Themen von EPHB2 / Hek5 Protein

Neuroscience>>Axon Guidance>>Ephrin & Eph Receptor>>EphB2

Cancer>>Growth Factor & Receptor>>Ephrin & Eph Receptor>>EphB2

Cancer>>Growth Factor & Receptor>>Receptor Tyrosine Kinase>>EphB2

Beschreibung von EPHB2 / Hek5 Protein

EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs). Ephrins are divided into the ephrin-A class, anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B class, the transmembrane proteins. The Eph receptor family are divided into two groups based on the similarity of their extracellular domain sequences and affinities for binding ephrin-A and ephrin-B ligands, making up the largest subgroup of the receptor tyrosine kinase (RTK) family. Human EphB2 cDNA encodes a 1055 aa precursor, which includes an 18 aa signal sequence, a 525 aa extracellar domain (ECD) containing a cysteine rich region followed by two fibronectin type I?II domains, a 21 aa transmembrane segment, and a 491 aa cytoplamic domain containing the tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif. It is expressed on both sides of the neuronal synapse controlling axon guidance across the embryonic midline, promoting a neuronal fate from neural precursors, and regulating NMDA receptor activity. EphB2 is a receptor for members of the ephrin-B family, and is proposed as a tumor suppressor. It is indicated that aberrant EphB2 expression and activity are implicated in the progression of several cancers.

References

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8. Merlos-Suarez A. et al., 2008, Curr Opin Cell Biol. 20: 194-200.