VEGF / VEGFA / VEGF165 Protein (Native)

16.11.2012

Sino Biological Inc. liefert VEGF / VEGFA / VEGF165 Protein (Native) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über VEGF / VEGFA / VEGF165 Protein (Native) lesen Sie bitte: http://www.sinobiological.com/VEGF-VEGFA-VEGF165-Protein-g-9868.html

Produkt-Information von VEGF / VEGFA / VEGF165 Protein

Synonym	VEGF165
Protein Construction	A DNA sequence encoding the mature form of human VEGF165 (Met 1- Arg191) was expressed.
Source	Human
Expression Host	Human Cells

QC Testing von VEGF / VEGFA / VEGF165 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: VEGF protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human VEGF165 consists of 165 amino acids and predicts a molecular mass of 19.2 KDa. It migrates as an approximately 26 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20 mM HAC-NaAC, 1M NaCl pH 6.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von VEGF / VEGFA / VEGF165 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Verwandte Produkte & Themen von VEGF / VEGFA / VEGF165 Protein

Related Areas:

Cancer>>Cancer Biomarkers>>VEGF

Cancer >>Growth factor & Receptor>>VEGF & Receptor>>VEGF

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
VEGF/VEGF-A	Human	VEGF 121 Protein, Recombinant, with Native	10008-HNAB
VEGF/VEGF-A	Human	VEGF 165 Protein, Recombinant, with Native	11066-HNAB
VEGF/VEGF-A	Human	VEGF 183 Protein, Recombinant, with Native	10009-HNAB
VEGF/VEGF-A	Mouse	VEGFA / VEGF164 Protein, Recombinant, with Native	50159-MNAB
VEGF/VEGF-A	Danio rerio (zebrafish)	VEGF / VEGFA / VEGF165 Protein, Recombinant, with Native	63004-ZNAB
VEGF/VEGF-A	Rat	VEGF164 / VEGFA Protein, Recombinant, with Native	80006-RNAB

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human VEGF/VEGF-A	WB, ELISA	VEGF/VEGF-A Antibody, Rabbit PAb	11066-RP01
Human VEGF/VEGF-A	WB, ELISA	VEGF/VEGF-A Antibody, Rabbit PAb (Antigen Affinity Purified)	11066-RP02
Human VEGF/VEGF-A	WB, ELISA	VEGF / VEGFA / VEGF165 Antibody	11066-R105
Human VEGF/VEGF-A	WB, ELISA	VEGF121 / VEGF-A Antibody	10008-RP01
Human VEGF/VEGF-A	WB, ELISA	VEGF121 / VEGF-A Antibody (Antigen Affinity Purified)	10008-RP02
Human VEGF/VEGF-A	WB, ELISA	VEGF 183 / VEGF-A Antibody (Antigen Affinity Purified)	10009-RP02

Beschreibung von VEGF / VEGFA / VEGF165 Protein

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. As its name implies, VEGF-A activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (stimulation monocyte/macrophage, neurons, cancer cells, kidney epithelial cells ). VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Alternatively spliced transcript variants of VEGF-A, encoding either secreted or cell-associated isoforms, have been identified. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform and is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. Human VEGF165 shares 88%, 96%, 95% and 93% aa sequence identity with mouse, porcine, canine and feline VEGF165, respectively.

References

Mattei MG. et al., 1996, Genomics. 32 (1):168-9.
Borgatti P. et al.,1998, Eur J Immunol. 27 (11): 2805-11.
Gengrinovitch S. et al., 1999, J Biol Chem. 274 (16): 10816-22.
Zachary I. et al., 2001, Cardiovasc. Res. 49: 568-81.
Robinson CJ. et al., 2001, J Cell Sci. 114: 853-65.
Lee P. et al., 2002, Proc Natl Acad Sci. 99: 10470-5.
Lee GY. et al., 2006, Protein expressed and Purification. 46: 503-9.