Endosulfine alpha / ENSA Protein (His Tag)

11.04.2013

Sino Biological Inc. liefert Endosulfine alpha / ENSA Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Endosulfine alpha / ENSA Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/ENSA-Endosulfine-alpha-Protein-Antibody-a-5766.html

Produkt-Information von Endosulfine alpha / ENSA Protein

Synonym	ENSA
Protein Construction	A DNA sequence encoding the mature form of human ENSA (O43768-1) (Met1- Glu121) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von Endosulfine alpha / ENSA Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: ENSA protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human ENSA consists of 136 amino acids and predicts a molecular mass of 15.2 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von Endosulfine alpha / ENSA Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Endosulfine alpha / ENSA Protein

Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

References

Ye M. et al., 2001, Genome Res. 10 (10): 1546-60.
Apiou F. et al., 1999, Diabetes 48 (9): 1873-6.
Lennon G. et al., 1997, Genome Res. 6 (9): 791-806.

ENSA (Endosulfine alpha) related areas, pathways, and other information