EIF-5A / EIF5 Protein (GST Tag)

24.12.2012

Sino Biological Inc. liefert EIF-5A / EIF5 Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über EIF-5A / EIF5 Protein (GST Tag) lesen Sie bitte: http://www.sinobiological.com/EIF-5A-EIF5-Protein-g-10176.html

Produkt-Information von EIF-5A / EIF5 Protein

Synonym	EIF5
Protein Construction	A DNA sequence encoding the mature form of human EIF5 (P55010) (Met1-Asp150) was fused with the GST tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von EIF-5A / EIF5 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: EIF-5A protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human EIF5 /GST chimera consists of 384 amino acids and has a predicted molecular mass of 44.1 kDa. It migrates as an approximately 38-43 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von EIF-5A / EIF5 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von EIF-5A / EIF5 Protein

EIF-5A, also known as EIF5, plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, EIF-5A interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. EIF-5A is also reported to form a complex with eIF2. It is a critical component of the eIF2regulatory complex that inhibits the activity of the guanine-nucleotide exchange factor (GEF) eIF2B. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.

References

Si K. et al., 1996, J Biol Chem. 71 (28): 16934-8.
Das S. et al., 1998, J Biol Chem. 272 (50): 31712-8.
Conte MR. et al., 2006, Biochemistry. 45 (14): 4550-8.