RIFK / RFK Protein (His Tag)

21.12.2012

Sino Biological Inc. liefert RIFK / RFK Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über RIFK / RFK Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/RIFK-RFK-Protein-g-10175.html

Produkt-Information von Flavokinase / RFK Protein

Synonym	RFK
Protein Construction	A DNA sequence encoding the mature form of human RFK (Q969G6) (Met1- His155) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von Flavokinase / RFK Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: Flavokinase protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human RFK consists of 170 amino acids and predicts a molecular mass of 19.5 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mMTris 10% glycerol PH8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Flavokinase / RFK Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Flavokinase / RFK Protein

Flavokinase belongs to the transferases family. It contains 220 amino acids. Its secondary structure contains 69 residues in alpha helix form, and 60 residues a beta sheet conformation. T Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase.

References

Hirano G. et al., 2011, Int J Oncol. 38 (4): 893-902.
Danielsen JM. et al., 2011, Mol Cell Proteomics. 10 (3): M110.003590.