EGFR / HER1 / ErbB1 Antikörper

10.05.2012

Produkt-Information von EGFR / HER1 / ErbB1 Antikörper

Immunogen	Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
Antibody Type	Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
Clone ID	043
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met 1-Ser 645).

Usage Guide von EGFR / HER1 / ErbB1 Antikörper

Specificity

Human EGFR / HER1 / ErbB1

No cross-reactivity in ELISA with

Human ErbB2

Human ErbB3

Immunochemistry

IHC-P: 1-10 μg/mL

IHC-Fr: 20-50 μg/mL

EGFR-placenta immunohistochemistry EGFR-uterine-cervix-cancer immunohistochemistry

Immunochemical staining of human EGFR in human placenta with rabbit monoclonal antibody (5 μg/mL,formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.

Immunochemical staining of human EGFR in human uterine cervix cancer with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to uterine gland.

EGFR-rectal-cancer immunohistochemistry

Immunochemical staining of human EGFR in human rectal cancer with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to rectal gland.
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

EGFR-skin-Fr immunohistochemistry

Immunofluorescence staining of human EGFR in human skin with rabbit monoclonal antibody (20 μg/mL, frozen section). The image showing membrane staining of squamous epithelium cell.

Western blot

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human EGFR in WB.

Direct ELISA

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EGFR.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hintergrund von EGFR / HER1 / ErbB1 Antikörper

As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is a type Ⅰ transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR signaling plays a crucial role in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, initiates subsequent tyrosine autophosphorylation and various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling has been shown to exert actions on carcinogenesis and disease progression, and thus EGFR was proposed as a target for cancer therapy currently.

References

Schlessinger, J., 2000, Cell. 103:211-225.
Giaccone, G., 2005, Ann. Onc. 16: 538-548.
Yarden, Y., 2001, Nat. Rev. Mol. Cell. Biol. 2: 127-137.

Sino Biological Inc. liefert EGFR / HER1 / ErbB1 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über EGFR / HER1 / ErbB1 Antikörper lesen Sie bitte: http://www.sinobiological.com/EGFR-HER1-ErbB1-Antibody-g-8779.html

EGFR / HER1 / ErbB1 Antikörper

Produkt-Information von EGFR / HER1 / ErbB1 Antikörper

Usage Guide von EGFR / HER1 / ErbB1 Antikörper

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Hintergrund von EGFR / HER1 / ErbB1 Antikörper

References