ECSIT Protein (His Tag)

28.05.2013

Sino Biological Inc. liefert ECSIT Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über ECSIT Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/ECSIT-Protein-Antibody-a-6205.html

Produkt-Information von ECSIT Protein

Synonym	ECSIT
Protein Construction	A DNA sequence encoding the human ECSIT (Q9BQ95-1)(Glu246-Ser431) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von ECSIT Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: ECSIT protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human ECSIT consists of 201 amino acids and predicts a molecular mass of 22.9 KDa. It migrates as an approximately 25 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von ECSIT Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ECSIT Protein

ECSIT is an adapter protein of the toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Activation of NF-kappaB as a consequence of signaling through the Toll and IL-1 receptors is a major element of innate immune responses. ECSIT is specific for the Toll/IL-1 pathways and is a regulator of MEKK-1 processing. It bridges TRAF6 to MEKK-1. Expression of wild-type ECSIT accelerates processing of MEKK-1, whereas a dominant-negative fragment of ECSIT blocks MEKK-1 processing and activation of NF-kappaB. ECSIT is also required for normal embryonic development and efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.

References

Kopp E. et al., 1999, Genes Dev. 13 (16): 2059-71.
The MGC Project Team. 2004, Genome Res. 14: 2121-7.
Vogel RO. et al., 2007, Genes Dev. 21: 615-24.