CFL1 / N-cofilin Protein (His Tag)

28.05.2013

Sino Biological Inc. liefert CFL1 / N-cofilin Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CFL1 / N-cofilin Protein (His Tag) lesen Sie bitte:http://www.sinobiological.com/CFL1-n-cofilin-Protein-Antibody-a-6202.html

Produkt-Information von CFL1 / N-cofilin Protein

Synonym	CFL1, CFL
Protein Construction	A DNA sequence encoding the human CFL1 (P23528) (Met1-Leu166) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von CFL1 / N-cofilin Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: CFL1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human CFL1 consists of 181 amino acids and predicts a molecular mass of 20.3 KDa. It migrates as an approximately 21 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von CFL1 / N-cofilin Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CFL1 / N-cofilin Protein

CFL1, also known as n-cofilin, is a member of the ADF/Cofilin family. This family comprises three genes: CFL1, CFL2 and DSTN (destrin). ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end. Cofilin also binds with other proteins such as myosin, tropomyosin, α-actinin, gelsolin and scruin. These proteins compete with cofilin for actin binding. Сofilin also plays a role in innate immune response. CFL1 contains 1 ADF-H domain and is widely distributed in various tissues. It is important for normal progress through mitosis and normal cytokinesis.

References

Lappalainen P. et al., 1997, Nature. 388 (6637): 78-82.
Ichetovkin I. et al., 2000, Cell Motil. 45 (4): 293-306.
Carlier MF. et al., 1997, J Cell Biol. 136 (6): 1307-22.