Dim2 / TXNL4B Protein (His Tag)

29.03.2012

Produkt-Information von Dim2 / TXNL4B Protein

Synonym	DLP, Dim2
Protein Construction	A DNA sequence encoding the human TXNL4B (Q9NX01) (Met 1-Ile 149) was expressed, with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von Dim2 / TXNL4B Protein

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: Dim2 / TXNL4B protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human TXNL4B consisting of 165 amino acids and migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions as predicted.
Formulation	Lyophilized from 0.2μm filtered solution of PBS, 20%glycerol, 0.1%Tween20, 50mM Arg; pH7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Dim2 / TXNL4B Protein Usage Guide

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Dim2 / TXNL4B

Dim2, also known as TXNL4B thioredoxin-like 4B,N-Histidine-tagged) is a member of the DIM1 family which has a vital role in pro-mRNA splicing. The Dim protein family is composed of two classes, Dim1and Dim2, which share a common thioredoxin-like fold. In spite of their biological and structural similarities, Dim1 and Dim2 proteins differ in many aspects. Dim1 bears distinctive structural motifs responsible for its interaction with other spliceosome components. Dim2 forms homodimers and contains specific domains required for its interactions with partners. This originality suggests that although both proteins are involved in pre-mRNA splicing, they are likely to be involved in different biological pathways. Dim2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 185 amino acids (1-149 a.a) and having a molecular mass of 21.1kDa. It is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Dim2 is required in cell cycle progression for S/G2 transition and has been shown to interact with PRPF6.

References

Sun X. et al., 2004, J Biol Chem. 279 (31): 32839-47.
Maruyama K. et al., 1994, Gene. 138 (1-2): 171-4.
Suzuki Y. et al., 1997, Gene. 200 (1-2): 149-56.

Sino Biological Inc. liefert Dim2 / TXNL4B Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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