BPHL Protein (His Tag)

14.03.2013

Sino Biological Inc. liefert BPHL Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über BPHL Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/BPHL-Protein-g-10605.html

Produkt-Information von BPHL Protein

Synonym	BPHL, MCNAA
Protein Construction	A DNA sequence encoding the mature form of human BPHL (Q86WA6-1) (Ser38-Gln291) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von BPHL Protein

Purity	> 95% as determined by SDS-PAGE.	SDS-PAGE: BPHL protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human BPHL consists of 268 amino acids and predicts a molecular mass of 30.7 KDa. It migrates as an approximately 31 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 20mM Tris, 50mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von BPHL Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von BPHL Protein

BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.

References

Lai L. et al., 2008, J Biol Chem. 283 (14): 9318-27.
Davila S. et al., 2010, Genes Immun. 11 (3): 232-8.
Hendrickson SL. et al., 2010, PLoS One. 5 (9): e12862.