DARS Protein (His Tag)

25.03.2013

Sino Biological Inc. liefert DARS Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über DARS Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/DARS-Protein-Antibody-a-5738.html

Produkt-Information von DARS Protein

Synonym	DARS
Protein Construction	A DNA sequence encoding the mature form of human DARS (P14868) (Met1-Pro501) was expressed with a polyhistide tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von DARS Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: DARS protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human DARS consists of 516 amino acids and predicts a molecular mass of 59 KDa. It migrates as an approximately 47 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile 50mM Tris, 100mM Nacl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von DARS Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von DARS Protein

Aspartate tRNA ligase 1, also known as DARS, is part of a multienzyme complex of aminoacyl-tRNA synthetases. It is a member of the class-II aminoacyl-tRNA synthetase family. Aspartate tRNA ligase 1 catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid(AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. It charges its cognate tRNA with aspartate during protein biosynthesis.

References

Escalante C. et al., 1993, J Biol Chem. 268 (8): 6014-23.
Maruyama K. et al., 1994, Gene. 138 (1-2): 171-4.
Reed VS. et al., 1995, J Biol Chem. 269 (52): 32932-6.