Cynomolgus Layilin / LAYN Protein

13.03.2013

Sino Biological Inc. liefert Cynomolgus Layilin / LAYN Protein mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Cynomolgus Layilin / LAYN Protein lesen Sie bitte: http://www.sinobiological.com/Layilin-LAYN-Protein-g-10585.html

Produkt-Information von Layilin / LAYN Protein

Synonym	LAYN
Protein Construction	A DNA sequence encoding the cynomolgus LAYN (Met1-Glu220) was expressed with the Fc region of human IgG1 at the C-terminus.
Source	Cynomolgus
Expression Host	Human Cells

QC Testing von Layilin / LAYN Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: Layilin protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 22
Molecular Mass	The recombinant cynomolgus LAYN is a disulfide-linked homodimer. The reduced monomer comprises 440 amino acids and has a calculated molecular mass of 49.8 KDa.The apparent molecular mass of the protein is approximately 58 KDa respectively in SDS-PAGE.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von Layilin / LAYN Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Layilin / LAYN Protein

Layilin, a recently characterized transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and to the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. All the above actions reveal an interesting parallel between layilin and the known HA receptor CD44. In addition, merlin and radixin have been identified as different intracellular binding partners of layilin. Accordingly, it has been suggested that layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction.

References

Borowsky ML. et al., 1998, J Cell Biol. 143: 429-42.
Bono P. et al., 2001, Mol Biol Cell. 12: 891-900.
Rees DJG. et al., 1990, Nature. 347: 685-9.
Bono P. et al., 2005, Exp.Cell. Res. 308: 177-87.
Schoenwaelder SM. et al., 1999, Curr Opin Cell Biol. 11: 274-86.